Solar powered biohydrogen production requires specific localization of the hydrogenase
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چکیده
منابع مشابه
Solar powered biohydrogen production requires specific localization of the hydrogenase.
Cyanobacteria contain a bidirectional [NiFe] hydrogenase which transiently produces hydrogen upon exposure of anoxic cells to light, potentially acting as a "valve" releasing excess electrons from the electron transport chain. However, its interaction with the photosynthetic electron transport chain remains unclear. By GFP-tagging the HoxF diaphorase subunit we show that the hydrogenase is thyl...
متن کاملSolar powered biohydrogen production requires specific localization of the hydrogenase† †Electronic supplementary information (ESI) available: Supplementary Fig. 1–12 and supplementary Table 1. See DOI: 10.1039/c4ee02502d Click here for additional data file.
Supplementary Figure 1. Chromosomal map of the hox operon in Synechocystis sp. PCC 6803. Schematic showing integration of the eGFP cassette at the C-terminus of hoxF. OriT(RK2) in the disruption cassette stands for origin of transfer and allows for conjugation. The cassette also includes a 19bp and 20bp primer site called P1 and P2 respectively. These are identical in all disruption cassettes. ...
متن کاملImproved production of biohydrogen in light-powered Escherichia coli by co-expression of proteorhodopsin and heterologous hydrogenase
BACKGROUND Solar energy is the ultimate energy source on the Earth. The conversion of solar energy into fuels and energy sources can be an ideal solution to address energy problems. The recent discovery of proteorhodopsin in uncultured marine γ-proteobacteria has made it possible to construct recombinant Escherichia coli with the function of light-driven proton pumps. Protons that translocate a...
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BACKGROUND Hydrogenases catalyze reversible reaction between hydrogen (H2) and proton. Inactivation of hydrogenase by exposure to oxygen is a critical limitation in biohydrogen production since strict anaerobic conditions are required. While [FeFe]-hydrogenases are irreversibly inactivated by oxygen, it was known that [NiFe]-hydrogenases are generally more tolerant to oxygen. The physiological ...
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ژورنال
عنوان ژورنال: Energy Environ. Sci.
سال: 2014
ISSN: 1754-5692,1754-5706
DOI: 10.1039/c4ee02502d